Journal: iScience

Article Title: Polyethylene terephthalate microplastics impair erectile function through macrophage mediated cGAS-STING ferroptosis

doi: 10.1016/j.isci.2026.115044

Figure Lengend Snippet: PET-MPs induce macrophage-mediated inflammation and contribute to ED (A) ELISA analysis of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α in CC tissue. (B) Western blot analysis shows the expression of IL-1β, IL-6, and TNF-α. (C) IF staining for CD68 (green) and PET particles (red) in penile tissue; scale bars, 50 μm. (D) M1 and M2 polarization of macrophages assessed by dual IF staining; scale bars, 50 μm. (E) ICP/MAP measurement in the clodronate + H-MP group and PBS + H-MP groups. (F) ELISA of IL-1β, IL-6, and TNF-α after macrophage depletion. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Equal amounts of protein (30 μg) were separated on 10% SDS-PAGE gels at 120 V for 2 h and transferred onto NC membranes at 400 mA for 1 h. Membranes were blocked with 5% non-fat milk at room temperature for 2 h and incubated overnight at 4°C with primary antibodies against TGF-β (Abcam, ab92486, 1:1000), α-SMA (Abcam, ab7817, 1:5000), eNOS (Cell Signaling Technology, CST #9572, 1:1000), p-eNOS (CST #9571, 1:1000), β-tubulin (Proteintech, 10068-1-AP, 1:5000), IL-1β (CST #12703, 1:1000), IL-6 (CST #12912, 1:1000), TNF-α (Abcam, ab6671, 1:1000), PPARα (Abcam, ab8934, 1:1000), PGC-1α (Abcam, ab54481, 1:1000), SDHB (Proteintech, 10620-1-AP, 1:2000), cGAS (CST #31659, 1:1000), STING (CST #13647, 1:1000), GPX4 (Abcam, ab125066, 1:1000), E-cadherin (CST #3195, 1:1000), Smad2 (CST #5339, 1:1000), Smad3 (CST #9523, 1:1000), p-Smad2 (CST #3108, 1:1000), p-Smad3 (CST #9520, 1:1000), CD31 (Abcam, ab28364, 1:1000), and FSP1 (Abcam, ab218163, 1:1000).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Staining